Opsin Expression in Human Epidermal Skin

Opsin Expression in Human Epidermal Skin

Human skin is constantly exposed to solar light containing visible and ultraviolet radiation (UVR), a powerful skin carcinogen. UVR elicits cellular responses in epidermal cells via several mechanisms: direct absorption of short-wavelength UVR photons by DNA, oxidative damage caused by long-wavelength UVR, and, as we recently demonstrated, via a retinal-dependent G protein-coupled signaling pat...

Isolation and in vitro cultivation of human skin Keratinocytes and preparation of epidermal sheet

Selective Modulation of Hedgehog/GLI Target Gene Expression by Epidermal Growth Factor Signaling in Human Skin

Epigenetic silencing of genes by hypermethylation of promoter sequences constitutes an important step in tumourigenesis and has equally been described as first or second hit. The hypermethylation of the Adenomatous Polyposis Coli (APC) promoter is a common event in a variety of tumours, for example in colon cancer or lung cancer. The APC protein is not only a key player in the wnt signalling pa...

Altered Expression of Epidermal Growth Factor Receptor (EGFR) in Glioma

      EGFR is a key molecule in cancer cells. EGFR signaling was shown to promote tumor cell proliferation and survival, invasion and angiogenesis and mediate resistance to treatment, including ionizing radiation in preclinical models. We extracted proteins from astrocytoma (III and IV) oligodendroglioma(IV) tumors and normal brain tissues and then evaluated the protein purity by Bradford test ...

Restoration of Vision with Ectopic Expression of Human Rod Opsin

Many retinal dystrophies result in photoreceptor loss, but the inner retinal neurons can survive, making them potentially amenable to emerging optogenetic therapies. Here, we show that ectopically expressed human rod opsin, driven by either a non-selective or ON-bipolar cell-specific promoter, can function outside native photoreceptors and restore visual function in a mouse model of advanced re...